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Macromolecular intracellular uptake

Important Issues on Nanomedicine Intracellular Uptake of Macromolecular Drugs, Drug Release Rate from the Complex, and Drug Resistance... [Pg.108]

In this chapter, we provide protocols to determine the ability of a peptide to mediate DNA internalization in cultured human tumor cells. Fluorescence-assisted cell sorting (FACS) analysis is used to obtain quantitative data on the time and temperature dependence of macromolecular delivery. Confocal microscopy is used to study the subcellular localization in both fixed and live cells. Fluorescently labeled transferrin and dextran are used to label the clathrin-dependent (15) and the non-clathrin, non-caveolar (16) endocytic compartments, respectively. Expression of a caveolin-l-YFP fusion protein is used to label cell surface caveolae and intracellular caveosomes (17). Finally a protocol, for the overexpression of dominant-negative dynamin [GTPase deficient dynamin-2 containing the amino acid substitution K44A (18)] is provided to evaluate the dynamin dependence of the uptake mechanism. [Pg.102]

See other pages where Macromolecular intracellular uptake is mentioned: [Pg.113]    [Pg.109]    [Pg.110]    [Pg.103]    [Pg.111]    [Pg.111]    [Pg.103]    [Pg.111]    [Pg.111]    [Pg.226]    [Pg.47]    [Pg.253]    [Pg.98]    [Pg.303]    [Pg.4]    [Pg.4]    [Pg.7]    [Pg.101]    [Pg.1370]    [Pg.112]    [Pg.334]    [Pg.8]    [Pg.147]    [Pg.390]    [Pg.432]    [Pg.215]    [Pg.101]    [Pg.714]   
See also in sourсe #XX -- [ Pg.111 ]

See also in sourсe #XX -- [ Pg.111 ]



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Intracellular uptake

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