Lysyl oxidase active site peptide

Kagan, H. M., and Cai, P. (1995). Isolation of active site peptides of lysyl oxidase. Methods Enzymol. 258, 122-132. [Pg.456]

The Isolation and Characterization of Active Site Peptides in Lysyl Oxidase... [Pg.351]

Lysyl oxidase contains a single Type 2 copper center (see Copper Proteins with Type 2 Sites) at the active site, bound by three histidine residues. The metal ion appears not to be redox active during turnover, and the oxidation chemistry is instead performed by a covalently attached cofactor, lysine tyrosyl quinone, derived from a tyrosine residue in the protein (vide infra. Section 3.1). The overall reaction involves oxidation of the primary amine group of a peptide-bound lysine residue, reduction of dioxygen to hydrogen peroxide, and release of ammonium ion (equation 4). [Pg.5498]