Lysozyme partitioning

FIGURE 14.5 Comparison of partition factors for a PAH (biphenyl) and polypeptide (lysozyme) versus percent acetonitrile concentration. 

TABLE 10 Partition Coefficient of Lysozyme and Catalase in Four Aqueous Two-Phase Systems... 

Fig. 36. Energy distributions of waters in different environments in the triclinic lysozyme unit cell, (a) All waters, total energy (-----) and energy partitioned into water-... 

The partition coefficients predicted by the theory for the four globular protein, lysozyme, chymotrypsin, albumin and catalase were determined by inserting into Equation 31 the second virial coefficients calculated for each... 

Figure 2. Predicted protein partition coefficient versus PEG molecular weight for lysozyme, chymotrypsin, albumin and catalase. Dextran molecular weight is 23,000 and polymer composition is PEG 6% Dx 8%.

From the observation that the partitioning behavior of each protein was not affected by the presence of the others (38, 49), Goklen and Hatton resolved a mixture of cytochrome-c, ribonuclease-A and lysozyme, three low molecular weight proteins comprised in the range 12.4 - 14.3 kDa. With the same reversed micellar phase, Woll et (49) showed that the selectivity of extraction between ribonuclease-A and concanavalin-A could be modulated by vaiying the surfactant concentration a 40% enhancement... 

The basic partitioning is illustrated schematically in Fig. 8a and realistically in Fig. 8b for a simulation study focusing on the dynamics of a tryptophan ring in the protein lysozyme.108 With the division indicated in the figure the total number of atoms to be simulated is 696 (294 protein atoms and 134 water molecules). This is a great reduction from the estimated 11,766 atoms (1266 protein atoms and 3500 water molecules) that would be necessary if conventional periodic boundary conditions were employed the estimate is based on using a 50-A cubic cell, a 26-A sphere to represent lysozyme, and 1 g/cm3 density for water. 

Figure 1 Partition of the lysozyme-substrate system into quantum mechanical and classical regions. The shaded area defines the quantum mechanical region, which is presented in greater detail on the right-hand side of the Figure. Only part of the enzyme is awn, although the whole enzyme is included in the calculation.

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