Lysolecithin, lysophosphatidylcholine

Lysolecithin (lysophosphatidylcholine) may be formed by an alternative route that involves lecithin cholesterol acyltransferase (LCAT). This enzyme. 

This enzyme [EC 2.3.1.23], also called lysolecithin acyl-transferase and lysophosphatidylcholine acyltransferase, catalyzes the reaction of an acyl-CoA derivative with 1-acyl-5 n-glycero-3-phosphocholine to yield coenzyme A and l,2-diacyl-5 n-glycero-3-phosphocholine. The enzyme preferentially acts on unsaturated acyl-CoA derivatives, but l-acyl-5 n-glycero-3-phosphoinositol can also act as the acceptor. 

The eluent is prepared just before the elution by adding solid lysophos-phatidylcholine (lysolecithin) to the buffered 1M GuHCl (pH should be checked and, if necessary, adjusted before the addition of lysophosphatidylcholine). 

Prepare 10 ml of permeabilization buffer NIM warmed to room temperature containing 0.5 mg/ml L-a-lysolecithin (a-lysophosphatidylcholine cat. L 4129 Sigma Chemical Co.), 1.0 ngjml leupeptin (cat. 1034 626 Boehringer-Mannheim Canada, Laval, QC), and 100 /xAf phenylmethylsulfonyl fluoride (PMSF, cat. 837 091 Sigma Chemical Co). Note Lysolecithin is poorly soluble in cold NIM. 

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