Lyases purification

Axley, M. J., Grahame, D. A., and Stadtman, T. C., 1990, Ecsherichia coli formate-hydrogen lyase. Purification and properties of the selenium-dependent formate dehydrogenase complex, J. Biol. Chem. 265 18213nl8218. 

Oka J, Ueda K, Hayaishi O et al. ADP-ribosyl protein lyase. Purification, properties and identification of the product. J Biol Chem 1984 259(2) 986-95. 

The authors wish to thank Maijo Searle-van Leeuwen for purification of the P-galactosidase from Aspergillus niger and Novo Nordisk Ferment Ltd Dittingen (Switzerland) for assistance in the purification of the RG-hydrolase and Novo Nordisk A/S Bagsvaerd (Denmark) for the gift of the crude recombinant RG-lyase. 

Pectin lyase from Fusarium oxysporum f. sp. radicis lycopersici purification and characterization... 

The objective of this work has been the purification of the pectin lyase with pi 9.20, which is the most abundant. 

Figure 5 shows the pattern of lyase isoenzymes along the purification process at first, three bands with lyase activity (pis 9.20, 9.00 and 8.65) were detected in the ammonium sulfate precipitate (B 1) in the peak eluted from the Superdex 75HR1030 column, only one band with lyase activity was detected, that correspond to the PNL with pi 9.20 (B 2), but more proteins were detected by silver staining (A 2). 

Griffiths ET, PC Harries, R Jeffcoat, PW Trudgill (1987b) Purification and properties of a-pinene oxide lyase from Nocardia sp., strain P18.3. J Bacteriol 169 4980-4983. 

Nagasawa T, H Ohkishi, B Kawakami, H Yamano, H Hosono, Y Tani, H Yamada (1982) 3-chloro-D-ala-nine chloride-lyase (deaminating) of Pseudomonas putida CR 1-1. Purification and characterization of a novel enzyme occurring in 3-chloro-D-alanine-resistant pseudomonads. J Biol Chem 257 13749-13756. 

Hasslacher, M., Schall, M., Hayn, M. et al. (1997) High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts. Protein Expression and Purification, 11, 61-71. 

The highest degree of purification was achieved with extracellular endopectate lyase from the anaerobic bacterium Clostridium fel-sineum.Mi A 225-fold purified preparation, homogeneous in disc electrophoresis, was obtained by precipitation with ethanol, and chromatography on CM-cellulose and on Sephadex G-200. Its molecular weight (determined by gel chromatography) was 105,000. 

A purification procedure including all of the separation principles then known led to a 156-fold purification of exopectate lyase of Clostridium multifermentans, but pectinesterase could not be re-moved.51,105... 

Adsorption on calcium pectate and calcium phosphate, and chromatography on DEAE-cellulose, were used for the purification of pectin lyase from Aspergillus fonsecaeus.253 Two forms of pectin lyase (having pH optima at 7.3 and 8.3) were isolated266 from the culture filtrate of Sclerotinia fructigena by chromatography on Seph-adex G-75 and CM-Sephadex C-50. 

SCHWIMMER, S., KJAER, A., Purification and specificity of the C-S lyase of Albizzia lophanta, Biochim. Biophys. Acta, 1960, 42, 316-324. 

Dacey JWH, Wakeham SG (1986) Oceanic dimethylsulfide production during zooplankton grazing on phytoplankton. Science 233 1314-1316 Davison IR, Pearson GA (1996) Stress tolerance in intertidal seaweeds. J Phycol 32 197-211 De Souza MP, Yoch DC (1996) Dimethylsulfoniopropionate lyase from the marine macroalga Ulva curvata purification and characterization of the enzyme. Planta 199 433—138 Dicke M, Agrawal AA, Bruin J (2003) Plants talk but are they deaf Trends Plant Sci 9 403-105... 

Lohse DL, Linhardt RJ (1992) Purification and characterization of heparin lyases from Flavobacterium heparinum. J Biol Chem 267 24347-55. 

Procion Rubine MX-B, Procion Yellow H-A, and Turquoise MX-G. These dyes have found use over the last few decades in the purification of a broad range of proteins and enzymes, including albumin, decarboxylases, glycolytic enzymes, hydrolases, lyases, nucleases, oxidoreductases, synthetases, and transferases [77,78], The first use of dye-ligand affinity chromatography was described by Staal et al. in 1971 [79], Since that time, it has become an extremely popular tool for enzyme and protein purification, with hundreds of such compounds having been isolated by this technique [3-6,76-79],... 

Jansen, R. Woker, M. R. Kula, Purification and protein characterization of hydroxynitrile lyase from Sorghum and Almond. Biotechnol. Appl. Biochem. 1992, 15, 90-99. 

The lyase from C. perfritif ens was purified to homogeneity by use of preparative gel-electrophoresis as the final purification step, thus allowing investigation of its molecular parameters.408 The average mo-... 

Y. Uchida, Y. Tuskada, and T. Sugimori, Purification and properties of W-acetylneuraminaie lyase from Escherichia coli, J. Biochem. 96 507 (1984). 

K. Aisaka, A. Igarashi, K. Yamagushi, and T. Uwajima, Purification, crystallisation and characterization of V-acety Incur animate lyase from Escherichia coli, Biochem. J. 276 541 (1991). 

Dc Angclis, M., Curin, A.C., McSweeney, P.L., Faccia, M., Gobbetti, M. 2002. Lactobacillus reuteri DSM 20016 Purification and characterization of a cystathionine gamma-lyase and use as adjunct starter in cheesemaking. J Dairy Res 69 255-267. 

S. Yu, L. Kenne, and M. Pedersen, a-l,4-Glucan lyase, a new class of starch/glycogen degrading enzyme. I. Efficient purification and characterization from red seaweeds, Biochim. Biophys. Acta, 1156 (1993) 313-320. 

Kobayashi, M., Nakagawa, H., Suda, I., Miyagawa, I., and Matoh, T., 2002, Purification and cDNA cloning of UDP-D-glucuronate carboxy-lyase (UDP-D-xylose synthase) from pea seedlings. Plant Cell Physiol. 43 1259-1265. 

Cabezas A, Pinto RM, Fraiz F Canales J, Gonzalez-Santiago S, and Cameselle JC (2001) Purification, characterization, and substrate and inhibitor structure-activity studies of rat liver FAD-AMP lyase (cyclizing) preference for FAD and specificity for splitting ribonucleoside diphosphate-X into ribonucleotide and a five-atom cyclic... 

Hughes, J., de Carvalho, F.J.P and Hughes, M.A. (1994) Purification, characterization and cloning of a-hydroxynitrile lyase from cassava (Manihot esculenta Crantz). Ardz. 

Wajant, H., Roerster, S., Selmar, D., Effenberger, R. and Pfizenmaier, K. (1995) Purification and characterization of a novel (R)-mandelonitrile lyase from the fern Phlebodium aureum. Plant Physiol, 109,1231-8. 

---