Lutein resonance Raman spectroscopy

Infrared and Resonance Raman Spectroscopy. Reviewson the uses of resonance Raman spectroscopy in biochemistry and biology include sections on carotenoproteins, visual pigments, and bacteriorhodopsin. The resonance Raman spectrum of the lowest excited triplet state of /3-carotene has been reported.A resonance Raman method has been used for the quantitative analysis of /3-carotene and lutein (20) in tobacco.The mechanism of carotenoid-protein interactions in the carotenoproteins ovoverdin and /3-crustacyanin has been investigated by resonance Raman spectroscopy. " 2 axanthin (24) has been used as a resonance Raman probe of membrane structure. " The resonance Raman spectra have been reported of all-frans-anhydrovitamin A (194), " /3-ionone, retinals, and Schiff bases.The technique has been used extensively to study... 

Near-infrared spectroscopy (NIRS) and resonance Raman spectroscopy are techniques with rapidity, simplicity, safety, and low operational costs and are nondestructive techniques. The results obtained were shown to have high equivalency with HPLC. Breima and Berardo compared NIRS and HPLC in the quantification of 8 carotenoids in maize (a- and p-carotene, a- and p-cryptoxanthin, isolutein, lutein, violaxanthin, and zeaxanthin) and found good correlation between HPLC values and NIRS estimates [53]. 

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