Luminescence cellular uptake

In addition, pyrazoyl-azaxanthone can also be used as a sensitizer of lanthanide complexes. An emissive terbium complex Tb-58 incorporating a pyrazoyl-l-aza-xanthonechromophore (Figure 13.25) exhibits cellular uptake and possesses a much lower sensitivity to excited state quenching. For example, Tb-58 was incubated for varying periods of time (from 1 to 12 h 50 or 100 xM complex) with CHO or NIH/3T3 cells. Examination of the loaded cells by luminescence microscopy revealed complex uptake, and localization within endosomes in the cytoplasm, presumably following receptor mediated endocytosis, but no tendency to nuclear localization [75]. 

The species [Re(2,2 -bipyridine)(3-ethynylpyridine)(CO)3](BF4) exhibits the characteristic luminescent properties and moderate cytotoxicity of this general class of compound. By attaching this species to a lipidated peptide known to increase cell permeability, enhanced cellular uptake of Re-myr-Tat (myr-Tat stands for myristoylated HIV-1 Tat peptide) compared with the model compound has been found. Moreover, cytotoxicity studies showed an increase in potency to a level comparable with cisplatin." ... 

Iridium metal compounds have shown some remarkable effects recently for luminescent properties such as very high photoluminescent quantum yields [27, 29, 44] and high stability [46]. Two reviews [2, 4] have recently reported on phosphorescent heavy-metal complexes (Re, Ru, Os, Ir, and Rh) as bioimaging probes, including their photophysical properties, cytotoxicity, and cellular uptake mechanisms [2], and on transition metals (Ir, Re, Ru) in fluorescent cell imaging applications such as uptake and toxicity [4], respectively. 

Gold(l) alkynyl derivatives containing the water-soluble PTA and DAPTA phos-phane ligands (see examples 34-36 in Figure 4.10) are cytotoxic in cancer cells and their cellular uptake was confirmed by fluorescence microscopy using the luminescent properties of these organometallics [117]. As expected, the complexes lack... 

Zebli, B. Susha, A. S. Sukhorukov, G. B. Rogach, A. L. Parak, W. J., Magnetic Targeting and Cellular Uptake of Polymer Microcapsules Simultaneously Functionalized with Magnetic and Luminescent Nanocrystals. Langmuir, 2005, 21, 4262-4265. 

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