Localization of Vascular Endothelial Growth Factor

Immunohistochemical Localization of Vascular Endothelial Growth Factor 

The following method is recommended for immunostaining vascular endothelial growth factor (VEGF) (Callagy et al., 2000). Breast cancer tissues (including the invasive 

FIGURE 1.6. The reactivity of vascular endothelial growth factor in the cytoplasm of tumor cells in an epithelial nest of biphasic mesothelioma. This reactivity is one of the most potent angiogenic cytokines. Courtesy of Eric van Marck and Samir Kumar-Singh. 

The sections are incubated in the rabbit polyclonal anti-VEGF (Santa Biotechnology, CA) and diluted 1 100 for 30 min at room temperature. Antigen-antibody reaction is detected using the biotin-streptavidin-based detection kit (Dako). The reaction is developed using DAB+hydrogen peroxide and counterstained with Mayer s hematoxylin. Exclusion of the primary antibody serves as a negative control. The results of this procedure are shown in Fig. 1.7. 

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Chen YS, Hackett SF, Schoenfeld CL, Vinores MA, Vinores SA, Campochiaro PA. Localization of vascular endothelial growth factor and its receptors to cells of vascular and avascular epiretinal membranes. Br J Ophthalmol 1997 81 919 926. 

Lutty GA, McLeod DS, Merges C, Diggs A, Plouet J. Localization of vascular endothelial growth factor in human retina and choroid. Arch Ophthalmol 1996 114 971-977. 

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