Living cells, mRNA extraction from

For the extraction of mRNAs from a living cell, the AFM was used as a nano-manipulator (03). As described previously, the AFM tip makes direct contact with the sample, and thus it is possible to insert the tip into a cell body by increasing applied force. For the penetration, there are two kinds of methods ... 

Our method using an AFM can be applied to extracting other biomolecules as well as mRNA from living cells. When we try to identify specific biomolecules on the cell surface, we can use modified AFM tips that interact with our interest. We introduced here the method for AFM tip modification (Fig. 9). 

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