Liver metal/protein ratios

Fractionation of beef liver shows an aggregation of zinc as glutamic dehydrogenase activity to protein ratio reaches its maximum value. Simultaneously, the metal protein ratio of all other elements studied decreases with increasing enzyme purity. The zinc/protein and activity/protein ratios become maximal in the third crystallization. 

Other Metal-Peptide and -Protein Interactions.—The determination of protein-bound trace elements in biological material by neutron activation analysis has been described Zn, Hg, Cu, and Se were accurately detected in human liver samples, provided that most of the element concerned was protein bound. An interaction of mercury with a protein or a protein-DNA complex has been invoked to explain the partitioning of the metal in euchromatin over heterochromatin (from mouse liver nuclei) by a 10 1 ratio. " Bovine retinas, isolated rod outer segments and emul-phogene extracts of rod outer segments have been shown to contain appreciable amounts of Zn ", Ca and the zinc levels being light sensitive. 

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