Listeria attachment

Takeuchi K, Matute C M, Hassan AN and Frank J F (2000), Comparison of the attachment of Escherichia coli 0157 H7, Listeria monocytogenes, Salmonella typhimurium and Pseudomonas fluorescens to lettuce leaves , Journal of Food Protection, 63 (10), 1433-1437. 

Alvarez-Dominguez, C., Vazquez-Boland, J. A., Carrasco-Martin, E., Lopez-Mato, P., and Leyva-Cobain, E. (1997). Host cell heparan sulfate proteoglycans mediate attachment and entry of Listeria monocytongenes, and the listerial surface protein ActA is involved in heparan sulfate receptor recognition. Infect. Immun. 65, 78-88. 

In order to avoid chemical compounds at all, it is also possible to apply a high voltage to kill microbes on surfaces. It was found that a direct current kills E. coli cells, probably by heat or by hydrogen peroxide formation [84], Microbial cells can be effectively killed by using pulsed electric fields (PEF), probably by frequently disturbing the cell membrane potential [85], PEF that was found to lower microbial cell numbers in food and drinks was also shown to effectively kill E. coli and Listeria innocua cells attached to polystyrene beads [86], This demonstrates the potential of applying this purely physical method to surfaces as well. 

Dutreux N, Notermans S, Wijtzes T et al. (2000) Pulsed electric fields inactivation of attached and free-living Escherichia coli and Listeria innocua under several conditions. Int J Food Microbiol 54 91-98... 

Figure 11.4a,b show the microcapsules as initially formed with Listeria monocytogenes and Staphylococcus aureus attached to the forming polyamide capsule wall. The bacteria, respectively rod and coccoid shaped, were clearly visible on the outside surface of the capsules when labelled with ethidium bromide, as evidenced by confocal optical sections. Variation of the initial bacterial concentration and... 

Habimana, O., Meyrand, M., Meylheuc, X, et al. (2009) Genetic features of resident biofilms determine attachment of Listeria monocytogenes. Appl Environ Microbiol 75, 7814-7821. 

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