Liquid Chromatography Reactive metabolites

Ma, S., and Subramanian, R. (2006). Detecting and characterizing reactive metabolites by liquid chromatography/tandem mass spectrometry. J. Mass Spectrom. 41 1121-1139. 

Soglia, J. R., Contillo, L. G., Kalgutkar, A. S., Zhao, S., Hop, C. E. C. A., Boyd, J. G., and Cole, M. J. (2006). A semiquantitative method for the determination of reactive metabolite conjugate levels in vitro utilizing liquid chromatography-tandem mass spectrometry and novel quaternary ammonium glutathione analogs. Chem. Res. Toxicol. 19 480-490. 

Baer et al. during 1986 to 1988. Urine was screened by methods in effect in the federal probation system THC metabolite and opiates were screened by EMIT and confirmed by high performance liquid chromatography (HPLC) at cutoffs of 100 and 500 ng/mL, respectively. Cocaine metabolites and PCP were screened by thin-layer chromatography (TLC) at cutoffs of 2000 and 500 ng/mL, respectively, and confirmed by GC. Hair testing was by RIA. The RIA procedures, validated by GC/MS, showed no false positives due to cross-reactivity effects. 

Ma, S. and Zhu, M., Recent advances in applications of liquid chromatography-tandem mass spectrometry to the analysis of reactive drug metabolites, Chem. Biol. Interact., 179(1), 25, 2009. 

Lohmann, W. and Karst, U., Generation and identification of reactive metabolites by electrochemistry and immobilized enzymes coupled on-line to liquid chromatography/ mass spectrometry, Anal. Chem., 79(17), 6831, 2007. 

Sleno, L. et al., Investigating the in vitro metabolism of fipexide Characterization of reactive metabolites using liquid chromatography/mass spectrometry, Rapid Commun. Mass Spectrom., 21(14), 2301, 2007. 

Zhu, M. et al., Detection and structural characterization of glutathione-trapped reactive metabolites using liquid chromatography-high-resolution mass spectrometry and mass defect filtering, Anal. Chem., 79(21), 8333, 2007. 

Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Identification of Reactive Metabolites Via Trapping Studies... 

Rousu T, Pelkonen O, Tolonen A. Rapid detection and characterization of reactive drug metabolites in vitro using several isotope-labeled trapping agents and ultra-performance liquid chromatography/time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 2009 23 843-855. 

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