Liquid chromatography polarimeters

All melting points were determined on a Kofler micro-melting point apparatus and were uncorrected. Optical rotation were measured with WZZ polarimeter at room temperature. IR spectra were recorded with 5DX-FI spectrometer, and NMR spectra with Unity-400 spectrometer. The FAB- and EI-MS were determined on a VG-7070E spectrometer. Gas liquid chromatography (GLC) was run a Shimaz GC-6A unit equipped with a flame ionization detector (FLD). Experimental conditions for sugar TMS ether column, 5% SE-52 on chromosorb W, 3mmX2m column temp. 179°C injection temp. 200 C carrier gas(N )... 

For a characterization within a series of compounds often polarimetry supersedes CD spectroscopy because the chosen concentration for its measurement is not restricted by a too high absorption. The broad acceptance of the polarimetric standardization of chiral compounds always has been a motivation for improvements of polarimeters. Especially for the daily routine work there is a requirement for instruments with high convenience (see section on Polarimetry and CD detectors in liquid chromatography). 

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