Liquid chromatography-mass interactions

DelTAversano, C., Eaglesham, G., Quilliam, M.A. (2004). Analysis of cyanobacterial toxins by hydrophilic interaction liquid chromatography-mass spectrometry. J. Chromatogr. A 1028 155-64. 

Xue, Y.J., Liu, J., and Unger, S., A 96-well single-pot liquid-liquid extraction, hydrophilic interaction liquid chromatography-mass spectrometry method for the determination of muraglitazar in human plasma, J. Pharm. Biomed. Anal., 41(3), 979, 2006. 

Table 4.1-2 Summary of biochemical analysis of purvalanol B-Protein interactions. Binding of proteins to immobilized purvalanol B but not to CDK-inactive-N6-methylated purvalanol B was evaluated by immunoblotting or liquid chromatography-mass spectrometry (for endogenous jurkat proteins). Enzyme assays were performed with purified enzymes and percentage inhibition of kinase activity observed with 1 pM purvalanol B...

Turrell E, Stobo L, Lacaze JP, Piletsky S, Piletska E (2008) Optimization of hydrophilic interaction liquid chromatography/mass spectrometry and developmtait of solid-phase extraction for the determination of paralytic shellfish poistming toxins. J AOAC Int 91 1372-1386... 

Figure 5.11. Generic approaches to identify interacting proteins within complexes. The complex is isolated from cells by affinity purification using a tag sequence attached to a protein known to be in the complex. Alternatively, the complex can be immunprecipitated with an antibody to one of the proteins in the complex. The proteins are resolved by polyacrylamide gel electrophoresis, proteolyzed, and the mass of the resulting peptides is determined by mass spectrometry. Alternatively, the proteins can be proteolyzed and the resulting peptides resolved by liquid chromatography. The peptide masses are then determined by mass spectrometry and used for database searching to identify the component proteins.

As has been pointed out, both entropic and enthalpic interactions affect the chromatographic behavior of macromolecules. They are adjusted to the required type of separation by selecting appropriate stationary and mobile phases. In a third mode of liquid chromatography of polymers, liquid chromatography at the critical condition (LCCC) (Entelis etal., 1985,1986 Pasch, 1997), the adsorptive interactions are fully compensated by entropic interactions. This mode is also referred to as liquid chromatography at the critical point of adsorption. Hence, TAS is equal to AH and therefore, AG becomes zero. K is 1 irrespective of molar mass and, consequently, homopolymer molecules of different molar masses coelute in one chromatographic... 

Van Nuijs ALN, Tarcomnicu I, Bervoets L, Blust R, Jorens PG, Neels H, Covaci A (2009) Analysis of dmgs of abuse in wastewater by hydrophilic interaction liquid chromatography-tandem mass spectrometry. Anal Bioanal Chem 395 819-828... 

---