Liquid chromatography—high-resolution applications

J. M. Colin, Some applications of a commercial light scattering detector for liquid chromatography, /. High Resolution Chromatogr., 8, 39, 2005. 

High performance liquid chromatography (HPLC) has been by far the most important method for separating chlorophylls. Open column chromatography and thin layer chromatography are still used for clean-up procedures to isolate and separate carotenoids and other lipids from chlorophylls and for preparative applications, but both are losing importance for analytical purposes due to their low resolution and have been replaced by more effective techniques like solid phase, supercritical fluid extraction and counter current chromatography. The whole analysis should be as brief as possible, since each additional step is a potential source of epimers and allomers. 

Despite the advances made in high-performance liquid chromatography in recent years, there are still occasionally applications in which conventional column chromatography is employed. These methods lack the sensitivity, resolution and automation of HPLC. They include the determination of urea herbicides in soil, polyaromatic hydrocarbons, carbohydrates, chloroaliphatic compounds and humic and fulvic acids in non-saline sediments. The technique has also been applied in sludge analysis, e.g. aliphatic hydrocarbons and carboxylic acids. 

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