Lipopeptide vaccine synthesis

Fig. 1. Structural representations and fidelity of synthesis of lipopeptide and nonlipidated peptides. (A) Structure of the lipopeptide vaccines palmitic acid, Pam. (B) HPLC chromatograms of purified lipopeptides. Analysis by HPLC was performed on a Waters HPLC System using a Vydac C4 column with 0.1% TFA in water and 0.1% TFA in acetonitrile as a gradient mobile phase. A flow rate of 1 mL/min was used at a gradient of 2%/min. Chromatograms were obtained by the detection of absorbance at a wavelength of 214 nm. (C) Mass spectral analysis of purified lipopeptides. Lipopeptides were analyzed by mass spectrometry using an Agilent 1100 Series LCM/MSD ion trap.

WiesmuUer K-H, Bessler WG, Jung G (1992) Solid phase peptide synthesis of lipopeptide vaccines eliciting epitope-specific B-, T-helper and T-killer cell response. Int J Pept Protein Res 40 255-260. 

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Synthesis of Toll-Like Receptor-2 Targeting Lipopeptides as Self-Adjuvanting Vaccines... 

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