Lipo-oligosaccharides structures

M. Gilleron, J. Vercauteren, and G. Puzo, Lipo-oligosaccharidic antigen from Mycobacterium gastri, complete structure of a novel C4-branched 3,6-didcoxy-alpha-jcy/o-hcxopyranosc, Biochemistry, 22 (1994) 1930-1937. 

Structures of Major Trehalose-Containing Lipo-oligosaccharides of Mycobacterium... 

PDMS of native LOS-I showed a [M + Na]+ ion at m/z 1823, confirming the presence of one of each of C15, C15 OH, and C25 fatty acids per molecule of LOS and fragment ions of mlz 245.6, 477.3, 706.9, 993.9, and 1282.4 supported the evidence for the structure of LOS-I, showing that the fatty acids are present on the terminal 2-O-methyl-glucose of the trehalose unit. Similarly, for the LOS from the unknown Mycobacterium, the location of the acyl functions was confirmed by using PDMS. Thus, a combination of alkylation and degradation techniques and PDMS may be used simultaneously to establish the nature and position of the acyl functions on the lipo-oligosaccharides. 

G. O. Aspinall, M. A. Monteiro, H. Pang, L. A. Kuijanczyk, and J. L. Penner, Lipo-oligosaccharide of Campylobacter lari strain PC 637. Structure of the liberated oligosaccharide and an associated extracellular polysaccharide, Carbohydr. Res., 279 (1995) 227-244. 

M. Lopez-Lara, J. D. J. van den Berg, J. E. Thomas-Oates, J. Glushka, B. J. J. Lugtenberg, and H. P. Spaink, Structural identification of the lipo-chitin oligosaccharide nodulation signals of Rhizobium loti. Mol. Microbiol. 15 621 (1995). 

L. Cardenas, J. Dominguez, C. Quinto, 1. Lopez-Lara, B. Lugtenberg, H. Spaink, G. Radeniaker, J. Haverkamp, and J. Thomas-Oates, Isolation, chemical structures and biological activity of the lipo-chitin oligosaccharide nodulation signals from Rhizobium etii. Plant Mol. Biol. 29 453 (1995). 

M. M. A. Olsthoorn, I. M. Lopez-Lara, B. O. Petersen, K. Bock, J. Haverkamp, H. P. Spaink, and J. E. Thomas-Oates, Novel branched nod factor structure results from a-(l->3) fucosyl transferase activity The major lipo-chitin oligosaccharides from Mesorhizobium loti strain NZP2213 bear an a-(l->3) fucosyl substituent on a nonterminal backbone residue, Biochemistry, 37 (1998) 9024-9032. 

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