Lipid transfer activity fluorescence

The Millipore human kinase panel consists of 236 wt kinases (including some lipid kinases fluorescence energy transfer technology is used for lipid kinases). They also provide assays for 40 mutant kinases and for 11 kinases with special substrates or in activated states. As with most catalytic assay suppliers, whole panel screens are typically done at one ATP concentration (10 pM ATP for Millipore), but assays at the ATP Km can be requested. For more information on the Millipore panel, see http //www.millipore.com/drugdiscovery/dd3/ kinaseprofiler. 

Energy transfer experiments with factor V, factors Va and VaLC. Similar experiments have been performed with another factor involved in prothrombin activation but which is not vitamin K-dependent, namely factor V. The experiments were done with PC-PS (50-50) mixture labelled with 5 % PDPC or PDPA, at a single phospholipid-to-protein molar ratio, = 530. Fluorescence quenching values are reported in Table III. They are of the same magnitude with both probes, for factor V, factor Va and for factor VaLC in the absence of calcium. We conclude, therefore, that no significant selectivity for a particular class of lipid occurs. 

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