Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Lipid peroxidation products spectra

Feng, Z., W. Hu, S. Amin, and M. S. Tang, 2003. Mutational spectrum and genotoxicity of the major lipid peroxidation product, frani-4-hydroxy-2-nonenal, induced DNA adducts in nucleotide excision repair-proficient and -deficient human cells, 42 7848-7854. [Pg.399]

It has been shown that lung macrophages from patients with systemic sclerosis (SS) produced the elevated levels of nitric oxide, superoxide, and peroxynitrite and expressed the enhanced level of iNOS [281], NAC administration reduced peroxynitrite production and might be possibly recommended for the treatment SS patients. Solans et al. [282] found the significant enhancement of lipid peroxidation in erythrocytes from SS patients. Cracowski et al. [283] showed that in vivo lipid peroxidation was enhanced in scleroderma spectrum disorders including SS and undifferentiated connective tissue disease. [Pg.935]

In the presence of iron, fragmentation of both the cyclopentane and endoperoxide rings can give rise to malondialdehyde (MDA), an ubiquitous product of lipid peroxidation [53-55]. MDA is the intended target of the thiobarbituric acid reactive substances (TEARS) assay commonly used to measure Upid peroxidation, although a broad spectrum of other lipid electrophiles are also detected by this assay. [Pg.35]

Guajardo and Mascharak have found that the iron complexes [Fe(PMA)] (n = 1, 2) (84, 85) shown in Fig. 23, which are synthesized as iron bleomycin analogues, promote facile lipid peroxidation in the presence of O2 or H2O2 [140]. Reaction of linoleic acid (59) with O2 catalyzed by 84 and 85 gives the 13-OOH product in the selectivity of 80 and 75%, respectively. In the reaction of arachidonic acid, (86), the 15-OOH product is also selectively formed (80%) by the two complexes. The peroxidation is also promoted by H2O2. As a possible intermediate, a low-spin (hydroperoxo)-iron(III) species, [(PMA)Fe -OOH], has been detected by X-band EPR. The EPR spectrum is identical to that of the activated bleomycin. The reaction has been explained in terms of the radical mechanism, which involves H atom abstraction from lipid (LH) by [(PMA)Fe -OOH]. Peroxidized linoleic acid (L-00 ) has been detected by UV absorption at 234 nm, and a chain propagation reaction by the peroxy radical to produce lipid hydroperoxide (L-OOH) has been proposed. [Pg.142]

Example 4-Hydroxynon-2-enal (4-HNE) is a major aldehydic product of lipid peroxidation (LPO), its products being indicators for oxidative stress. In order to introduce LPO products as biomarkers, a GC-MS method for 4-HNE detection in clinical studies [35] was developed using a sample volume of 50 pi of plasma. For improved GC separation and subsequent mass spectral detection the aldehyde is converted into the pentafluorobenzyl-hydroxylimine and the hydroxy group is tri-methylsilylated [36]. The TIC acquired in electron capture mode (EC, Chap. 7.4) exhibits 50 chromatographic peaks (Fig. 14.2). Those related to the target compounds can easily be identified from suitable RICs. The choice of potentially useful m/z values for RICs is made from the EC mass spectrum of the pure 4-HNE derivative (below). In this case, [M-HF], m/z 403, [M-HOSiMes] , m/z 333, and [CeFs]", m/z 167, are indicative, while [CH2C6F5] , m/z 181, is not. [Pg.654]


See other pages where Lipid peroxidation products spectra is mentioned: [Pg.192]    [Pg.35]    [Pg.236]    [Pg.213]    [Pg.477]    [Pg.3947]    [Pg.233]    [Pg.491]    [Pg.377]    [Pg.78]    [Pg.84]    [Pg.161]    [Pg.7]    [Pg.281]    [Pg.25]    [Pg.164]   
See also in sourсe #XX -- [ Pg.85 ]

See also in sourсe #XX -- [ Pg.85 ]




SEARCH



Lipid peroxidation products

Lipid peroxide

Lipid production

Lipids peroxidation

Spectra product

© 2024 chempedia.info