Lipases and Acyl Transferases

Chitin deacetylase has been used in the synthesis of partially and fully N-deacetylated 4-methyl umberlliferyl chitobiose derivatives as fluorogenic substrates for chitinase. Conversely, the reverse acylating action of chitin deacetylase has been used to prepare a partially N-acetylated chitosamine tetramer. The preparation of a 4-0-acetyl sialic acid derivative by a lipase OF-catalysed deacetylation of the peracetylated precursor has been reported.  

Regioselectively protected cytidine derivatives have been prepared from the peracetylated nucleoside using lipase and esterase reactions. There have been reports on the use of bacterial proteases for the selective acylation of sucrose to produce a variety of different acyl and acrylate esters. 8-Aminooctyl 5-5-coniferyl-5-thio-a-L-arabinofuranoside(27) attached to sepharose proved to be a selective affinity ligand for feruloyl esterase A. niger  

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