Lipases selectivity

Henderson AD, Richmond W, Elkeles RS (1993) Hepatic and lipoprotein lipases selectively assayed in postheparin plasma. Clin Chem 39 218-223... 

Lipase-selective esterification has recently helped fractionate PUFA species present in the same mixture. Fish oil-derived FFA was esterified with glycerol using Lipozyme IM in solvent-free media to fractionate DHA and EPA. The unesterified FFA contained 78%o DHA and only 3% EPA, with a 79%o recovery of DHA the esters contained the majority of the EPA and the other acyl groups, with a 91%o recovery of EPA.f Lipases have also successfully fractionated the two most abundant species of CLAs 18 2-9c,llt and 18 2-10t,12c from an equimolar mixture derived from acid-catalyzed isomerization of ALA. ° ... 

Diks, R.M.M. Bosley, J.A. The exploitation of lipase selectivities for the production of acylgly-cerols. In Enzymes in Lipid Modification Bornscheuer, UT, Ed. Wiley-VCH Weinheim, Germany, 2000 3-22. 

In a screening procedure for characterization of lipase selectivity, Bradoo et al. [90] esterified sucrose and ascorbic acid with different fatty acids in a microwave oven using porcine pancreas, B. stearothermophilus SB-1 and B. cepacia RGP-10 lipases. Microwave-assisted enzyme catalysis was found to be an attractive procedure for rapid characterization of a large number of enzyme samples and substrates this would otherwise have been a cumbersome and time-consuming exercise. 

Halldorsson, A., Kristinsson, B., and Haraldsson, G.G., 2001, Lipase selectivity toward fish oil fatty acids, Lipidforum, 21stNordic Lipid Symposium, Bergen, June 5-8. 

Unfortunately, sueh formulas have resulted in poor absorption of fats and minerals, particularly when studied in infants dining the first few weeks of life [62, 91, 92]. This is because PA is present in the sn-1 position (human breast milk, lard native, enzyme-direeted and randomly ehemieally interesterified fats plant) eompared with the sn- and sn-3 positions (bovine milk, randomly ehemieally interesterified lard or crude palm oil). It was found the relative absorption of palmitic acid and full fat was linearly related to the proportion of palmitic acid in the sn-1 position of the TAG in human infants. Panereatie lipase selectively hydrolyses the fatty aeids at the sn- and sn-3 positions, yielding free fatty acids and monoacylglycerols (MGs). The TAG sn-1 position is absorbed more effieiently than free palmitic acid and it is conserved through the digestion, absorption and ehylomicron TAG synthesis [63, 91]. 

Table 3 lists some information concerning lipase selectivity important for the synthesis of sTAG. In addition to the selectivities listed, discrimination between lipid enantiomers by lipases is possible. Chandler et al (1998) used lipases from Rhizomucor miehei and Rhizopus delemar for the synthesis of... 

Papaya latex lipase Selective for sn- and sn-3 positions and for Gandhi and... 

---