Light scattering microspectroscopy

Raman microspectroscopy was not a completely new concept. In 1966, Delhaye and Migeon [35] showed that a laser beam could be hghtly focused at a sample, and that Raman-scattered light could be collected and transferred to a spectrometer, with minimal loss. Their calculahons showed that the increased irradiance more than compensated for the decrease in the size of the irradiated volume. The first Raman microscope was reported by Delhaye and Dhamelincourt in 1975 [36], and an instrument based on these principles (the MOLE) was introduced by Jobin Yvon at about the same time. However, the optical scheme used for imaging, which employed global illumination, was inefHcient and it was not until the advent of CCD-Raman spectrometers that the advantages of Raman microscopy became apparent. 

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