Light microscopy immunolabelling

JacksonP, Blythe D. Immunolabeling techniques Lor light microscopy, in Immunocytochemistry, A Practical Approach (Beesley JE, ed.), Oxford University Press, Oxford, UK, 1993, pp. 15-41. 

Immunohistochemical labeling for electron microscopy is based on the same principles as immunohistochemistry for light microscopy. The differences are that specimen sections must be much thinner (50 100 nm) and the label must be electron-dense. The first electron-dense labels used for immunolabeling at the electron microscope level were ferritin and peroxidase. Peroxidase label can be visualized using DAB reaction product which becomes electron-dense after osmi-cation. With the advent of colloidal gold particles as markers in immunocytochemical... 

Immunolabeling for electron microscopy is theoretically identical to immunolabeling for light microscopy. The problems discussed in Chapter 30 describing light microscope immunolabeling are entirely relevant to electron immunocytochemistiy. 

Bonnard, C., Papermasteg D.S., and Kraehenbuhl, J.-P. (1984) The streptavidin-biodn bridge technique Application in light and electron microscope immunocytochemistry. In Immunolabelling for Electron Microscopy, (J.M. Polak, and I.M. Varndell, eds.), pp. 95-111. Elsevier, New York. 

Roth, J. (1984) The protein A-gold technique for antigen localization in tissue sections by light and electron microscopy, in Immunolabelling for Electron Microscopy (Polak, J. M. and Vamdell, I. M., eds.), Elsevier, New York, pp. 113-122. 

C. Bonnard, D. S. Papermaster, and J.-P. Kraehenbuhl, The Streptavidin-biotin Bridge Technique Application in Light and Electron Microscope Immunocytochemistry, in Immunolabeling for Electron Microscopy (eds. J. M. Polak and I. M. Varndell), Elsevier, Amsterdam, 1984, pp. 95-111. 

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