Ligation glycoproteins

Staudinger ligation and its traceless version have been explored in the preparation of glycoconjugates [70, 71]. It was used to label cell-surface carbohydrates [72, 73] and, more recently, to prepare glycoproteins by modification of L-azidohomoalanine-containing proteins [74, 75],... 

Tolbert TJ, Franke D, Wong CH. A new strategy for glycoprotein synthesis ligation of synthetic glycopeptides with truncated proteins expressed in E. coli as TEV protease cleavable fusion protein. Bioorg. Med. Chem. 2005 13 909-915. 

Shin, Y, Winans, K A, Backes, B J, Kent, S B. H, EUman, J A, Bertozzi, C R, Fmoc-based synthesis of peptide- thioesters apphcation to the total chemical synthesis of a glycoprotein by native chemical ligation, J. Am. Chem. Soc., 121, 11684-11689, 1999. 

Glycoprotein Glycosylation CHO cell Yeast Plant E. colv. Chemical ligation... 

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