Ligation-dependent probe amplification

Wang J, Ban MR, Hegele RA (2005) Multiplex ligation-dependent probe amplification of LDLR enhances molecular diagnosis of familial hypercholesterolemia. J Lipid Res 46 366-372... 

Genomic quantification is necessary to identify large deletions or duplications that occasionally cause single gene disorders. A novel molecular method (multiplex ligation-dependent probe amplification, MLPA) has been recently developed for this purpose and is presented in this chapter. 

Fig. 8.2.2 Principle of genomic quantification by multiplex ligation-dependent probe amplification (MLPA). Synthetic oligonuleotides are designed to bind to exon 1 (oligo 1a and 1b) and 2 (2a and 2b). After specific hybridisation, the oligos are ligated. The ligation products undergo quantitative polymerase chain reaction using universal primers X and Y...

MLPA multiplex ligation-dependent probe amplification... 

Schouten JP, McElgunn CJ, Waaijer R, Zwijnenburg D, Diepvens F, Pals G. Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification. Nucleic Acids Res 2002 30(12) e57. 

Both Sanger and NGS have a limited ability to detect large deletions and duplications. Identification of such changes generally necessitates the use of other techniques. Options include array comparative genomic hybridization (aCGH) analysis, multiplex ligation-dependent probe amplification (MLPA) analysis, and quantitative PCR (qPCR) analysis. All three techniques allow... 

Zhang DY, Brandwein M, Hsuih TC, etal. Amplification of target-specific, ligation-dependent circular probe. Gene 1998 211 277-285. 

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