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Ligands SELEX

In the DCC SELEX screen, aldehydes, the TAR RNA target, and a random library of 2 -amino RNAs were allowed to equilibrate. Next, the TAR RNA target and bound ligands were separated from the aptamer library. The selected 2 -amino RNAs that bound the TAR RNA target were then reverse transcribed into DNA and PCR amplified. These double-stranded... [Pg.104]

Stoltenburg, R. Reinemann, C. Strehlitz, B. SELEX - A (r)evolutionary method to generate high-affmity nucleic acid ligands. Biomol. Eng. 2007, 24, 381 03. [Pg.117]

For cloning and sequencing of individual RNA aptamers, nine SELEX cycles are necessary to obtain high-affinity RNA ligands for the nAChR. [Pg.34]

Although its use in ligand selection for large scale of affinity chromatography is not wide, ribosome display and systematic evolution of ligands by exponential enrichment (SELEX) may... [Pg.73]

SELEX is a widely used technique for screening of aptamers which are nucleic acid ligands. According to this method, a pool of DNA with a random sequence region attached to a constant chain is constituted by amplification then transcribed to RNA. RNA pool is separated according to the affinity of RNA molecules to a target protein. DNA molecules obtained by reverse transcription from retarded RNA molecules are amplified and the cycle is repeated. [Pg.74]

Figure 10.17 Schematic representation of the systematic evolution of ligands by exponential (SELEX) enrichment process. (See the color version of this figure in Color Plates Section.)... Figure 10.17 Schematic representation of the systematic evolution of ligands by exponential (SELEX) enrichment process. (See the color version of this figure in Color Plates Section.)...
Figure 10.17 Schematic representation of the systematic evolution of ligands by exponential (SELEX) enrichment process. Figure 10.17 Schematic representation of the systematic evolution of ligands by exponential (SELEX) enrichment process.
SELEX (systematic evolution of ligands by exponential enrichment) is used to generate aptamers, oligonucleotides selected to tightly bind a specific molecular target. The process is generally automated to allow rapid identification of one or more aptamers with the desired binding specificity. [Pg.1030]

Aptamers are nucleic acids which exhibit a defined structure due to their nucleotide sequence and therefore, are able to specifically bind selected targets [1] (aptus [lat.] = fitting, sticking to). Aptamers and likewise, ribozymes [2] and deoxyribozymes [3] are selected in vitro by screening nucleic acid libraries. Here we describe in detail the selection of aptamers by a process called SELEX (Systematic Evolution of Ligands by Exponential enrichment) [4]. [Pg.65]

Fig. 2.5 (a) An aptamer-target protein interaction (adapted from Tuerk and Gold 1990, p. 505). (b) Principle of the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) process (adapted from Tombelli et al., 2005, p. 2424)... [Pg.23]

B.23 SELEX Systematic Evolution of Ligands by Exponential Enrichment 433... [Pg.433]


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