Ligand binding analysis

LIGAND BINDING ANALYSIS Ligand binding model for positive coopera-tivity,... 

NUCLEAR MAGNETIC RESONANCE LARMOR PRECESSION LIGAND BINDING ANALYSIS LINE-SHAPE ANALYSIS LOW-BARRIER HYDROGEN BONDS ROLE IN CATALYSIS MAGNESIUM ION (INTRACELLULAR) MAGNETIZATION TRANSFER Nuclear pores,... 

Flexible 3D alignment of a set of ligands binding to the same target and/or CoMFA analysis allowing the perception of a pharmacophore for this target. 

Analysis of Ligand Binding in the Intact Cell. A model of LR dynamics in the intact cell consistent with the results of these studies is presented in Figure 6, and a fit to the data of a polarization binding experiment is shown. The model has these characteristics ... 

Middle panel The data are a continuous polarization analysis of ligand binding (1 nW FLPEP) to lO cells/mL at 37 C, which represents LR.j,, the sum of occupied receptors in all... 

APPENDIX REAL-TIME METHODS FOR ANALYSIS OF LIGAND BINDING Method 1. Spectrofluorometric Method Using Antibody to Fluorescein... 

Csilibrsition. Quantitative binding analysis requires knowing the concentration of FLPEP, which can be determined for a stock solution of FLPEP by absorption spectroscopy. The quenching by the antibody is essentially quantitative, and the relative amounts of free and bound ligand are calculated from the relative fluorescence intensity. 

Robertson D., Benyon R.L. and Evershed R. (1993). Extraction, characterization and binding analysis of two pheromonally active ligands associated with major urinary proteins of house mouse (Mus musculus). J Chem Ecol 19, 1405-1416. 

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