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Ligand and Biomimetic Affinity Chromatography

FIGURE 1.3 Structures of Cibacron Blue 3GA and Procion Blue, two common binding agents used in dye-ligand affinity chromatography [52]. [Pg.10]

Labrou, N.E. Mazitsos, K. Clonis, Y.D. Dye-ligand and biomimetic affinity chromatography. In Handbook of 20. [Pg.36]

Lowe, C. R., Burton, S. I, Burton, N. P., Alderton, W. K., Pitts, J. M., and Thomas, J. A. (1992) Designer dyes-biomimetic ligands for the purification of pharmaceutical proteins by affinity-chromatography. Trends Biotechnol. 10, 442-448. [Pg.61]

Further examples of separation techniques that exploit the asymmetric distribution of amino acid residues at the surface of folded proteins include metal ion affinity chromatography (HP-IMAC), ligand exchange chromatography (HP-LEC), immunoaffinity chromatography (HP-IAC), hydrophilic chromatography (HP-HILIC), and the various modes of biospecific (HP-BAC), and biomimetic (HP-BMC) chromatography. For example, the... [Pg.119]

Partition 5 Biomimetic Ligands for Affinity Chromatography 63 1. Protein solution to be tested and the immobilized ligand to be... [Pg.63]

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