Leuconostoc mesenteroides synthesis

M. Remaud-Simeon, A. Lopez-Munguia, V. Pelenc, F. Paul, and P. Monsan, Production and use of glucosyltransferases from Leuconostoc mesenteroides NRRL B-1299 for the synthesis of oligosaccharides containing a-(l >2) linkages, Appl. Biochem. Biotechnol., 44 (1994) 101-117. 

Synthesis of Branch Linkages in Leuconostoc mesenteroides B-512F Dextran... 159... 

Kitao, S., Ariga, T., Matsudo, T., and Sekine, H. 1993. The synthesis of catechin-glucosides by transglycosylation with Leuconostoc mesenteroides sucrose phosphorylase. Biosci. Biotechnol. Biochem., 57, 2010-2015. 

Dols M, Remaud-Simeon M, Willemot RM, Demuth B, Jordening HJ, Buchholz K, Monsan P (1999) Kinetic modeling of oligosaccharide synthesis catalyzed by Leuconostoc mesenteroides NRRL B-1299 dextransucrase. Biotechnol Bioeng 63 308-315... 

G-6-P-DH from Leuconostoc mesenteroides is used in organic synthesis for example to synthesize D-lactic acid or (S)-benzyl alcohol [43]. Commercially available G-6-P-DH was coupled with ADH from Lactobacillus kefir for the regeneration of NADPH to produce optically pure (/ )-phenylethanol [33]. 

VedyashkinaTA, RevinW, Gogotov IN. Optimizing the conditions of dextran synthesis by the bacterium Leuconostoc mesenteroides grown in a molasses-containing medium. Appl Biochem Microbiol 2005 41 361-4. 

A reducing disaccharide, consisting of D-glucose and D-fructose, named levjcrose 214) is formed in the reaction mixture to the extent of about 3 % during the synthesis of dextran from sucrose by an enzyme isolated from the microorganism Leuconostoc mesenteroides. Its specific rotation is [a]o —6.8° (in water) m.p. 161-162°C. Methylation studies show that its structure is 5-0-a-D-glucopyranosyl-D-fructopyranose. 

Enzyme-catalyzed organic synthesis NAD(P)H cofactor regeneration by using glucose 6-phosphate and the glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides. J. Am. Chem. Soc., 103, 4890-4899. 

Deamidation of Nicotinic Add. Enzymes from microorganisms (185-188), insects (189), and birds have been found to deamidate nicotinamide to nicotinic acid (190). Recently, Sundaram et al. (191) have shown that a strain of Leuconostoc mesenteroides which will grow on nicotinic acid but not on nicotinamide, does not possess the deamidase and will not convert the pyridine amide to DPN. On the other hand, Saccharomyces cerevisiae will grow on both pyridine compounds, and the yeast contains the deamidase. These observations suggest that the route of synthesis of DPN from nicotinamide may involve the Preiss-Handler pathway, and that deamidation of nicotinamide occurs during the synthesis, which is also indicated by studies in the intact mouse (see Section III, A). 

Evidence has been presented that the synthesis of a-(l -> 3)-branch linkages in Leuconostoc mesenteroides B-512 dextran occurs by nucleophilic attack of a hydroxy-group at C-3 of a free dextran molecule on C-1 of the reducing-end of a dextran-dextransucrase complex the dextran is displaced from the complex on forming an a-(l -> 3)-linkage. Thus, the synthesis of branched linkages does not require a separate branching enzyme. 

Alcalde, M., Plou, F. J., Gomez de Segura, A., Remaud-Simeon, M., WUlemot, R. M., Monsan, P. Ballesteros, A. (1999). Immobilization of native and dextran-free dextransucrases from Leuconostoc mesenteroides NRRL B-512F for the synthesis of glucooligosaccharides Biotechnology Techniques, 13, 749-755. 

Glucansucrases are elaborated by Lactobacilli (Leuconostoc and Streptococcus species) and catalyze the synthesis of glucans from sucrose. In 1954, Jeanes et al. (1) surveyed 96 L. mesenteroides species that produce glucans from sucrose and found that the glucans... 

The synthesis of dextran from sucrose by a cell-free bacterial culture filtrate was first reported by Hehre in 1941 [152]. The genera of bacteria that are recognized to produce enzymes capable of synthesizing polysaccharides from sucrose are principally Leuconostoc and Streptococcus. These genera are gram positive, facultatively anaerobic cocci that are very closely related to each other. One notable difference between them is that the L. mesenteroides strains required sucrose in the growth medium to induce the formation of the enzyme(s), whereas the Streptococcus species did not require sucrose in the medium to form the enzymes [153]. 

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