Leuconostoc carnosum

DCH and 15 PS samples were inoculated with a mixed suspension of L. monocytogenes at a final concentration of 1,000 CFU/cm. After 1 h, a snspension of Leuconostoc carnosum (Christian Hansen, Italy) was plated on the same inoculated samples. The final concentration was 100,000 CFU/g. All samples were packaged under vacuum in PET, split into three groups, and stored at 4°C. The first group of each was analyzed at 0 days, the second at 15 days, and the third at 30 days in order to assess the behavior of L. monocytogenes. 

The effect of Leuconostoc carnosum 4010 (CHR Hansen, Italy) is reported in Table 8. As shown, the concentration of L. monocytogenes decreases to <1 CFU/g. Indeed, enrichment cultures managed to cut the concentration of the inoculated microorganism to less than 0 CFU/25 g. In other words, it seems that, after 30 days, protective cultures manage to achieve the 0 tolerance level recommended by FDA/ FSIS (2003b). 

Table 8 Behaviour of Leuconostoc carnosum CH versus Listeria monocytogenes in Data UFC/cm ...

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