Lead optimization, integrated index

As for enzyme-coupled reaction system, initial rate itself is estimated by kinetic analysis of reaction curve based on numerical integration and NLSF of calculated reaction curves to a reaction curve of interest. Consequently, neither the conversion of indexes nor the optimization of parameters for such conversion is required and the integration strategy can be realized easily. By kinetic analysis of enzyme-coupled reaction curve, there still should be a minimum number of the effective data and a minimum substrate consumption percentage in the effective data for analysis these prerequisites lead to unsatisfactory lower limits of linear response for favourable analysis efficiency (the use of reaction duration within 5.0 min). The classical initial rate method is effective to enzyme-coupled reaction systems when activities of the enzyme of interest are not too high. Therefore, this new approach for kinetic analysis of enzyme-coupled reaction curve can be integrated with the classical initial rate method to quantify enzyme initial rates potentially for wider linear ranges. 

Another class of polarizability formulations is based not on the integral equation in Eq. (2.5) but on the notion of a set of point dipoles. Draine and Goodman [100] found an optimal 0 ((W) ) correction to the CM polarizability in the sense that an infinite lattice of point dipoles with such polarizability would lead to the same propagation of a plane-wave as in a homogeneous medium with a given refractive index. This polarizability was called the lattice dispersion relation (LDR) ... 

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