LC Selectivity for Peptides Experimental Design

In an effort to identify promising LC modes for 2DLC separation of tryptic peptides several traditional, as well as novel, LC modes have been evaluated, as earlier reported 

Analysis using reversed-phase chromatography at low pH was carried out under conditions typically used for 2DLC-MS/MS analysis of peptides. The mobile phases for other chromatographic conditions were also chosen to be compatible with MS detection, including SCX LC, where the peptides were eluted with volatile ammonium formate buffer. 

TABLE 12.1 Chromatographic Modes and Conditions Used for LC-MS Study of Tryptic Peptides Separation Selectivity 

LC mode Column type Column L x i.d dp. (mm x mm pm) Elution conditions0 

HILIC Atlantis HILIC 150x2.1 3 lOmM ammonium formate, pH 4.5, 904-8% acetonitrile in 50 min 

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