Large-scale protein identification

Wilkins MR et al (1996) From proteins to proteomes large scale protein identification by two-dimensional electrophoresis and amino acid analysis. BioTechnol 14 61-65... 

Lin D,Tabb DL, Yates JR. Large-scale protein identification using mass spectrometry. Biochim. Biophys. Acta. 2003 1646 1-10. 

Wilkins MR, Pasquali C, Appel RD, Ou K, Golaz O, Sanchez JC, Yan JX, Gooley AA, Hughes G, Humphery-Smith I, Williams KL, Hochstrasser DF. 1996. From proteins to proteomes Large scale protein identification by two-dimensional electrophoresis and amino acid analysis. Biotechnology (NY) 14 61-65. 

From proteins to proteomes - large-scale protein identification by 2-dimensional electrophoresis and amino-add-analysis. Bio-Technology 14, 61-65. 

Barile, M., Pisitkun, X, Yu, M.J., Chou, C.L., Verbalis, M.J., Shen, R.F. and Knepper, M.A. (2005) Large scale protein identification in intracellular aquaporin-2 vesicles from renal inner medullary collecting duct. Mol. Cell Proteomics 4, 1095-1106. 

The introduction of delayed ion extraction in MALDI-TOF instruments in 1995 resulted in a tremendous improvement in analytical performance, mass resolution and accuracy. Using this technique, peptide mass mapping gained high specificity for protein identification and an ability to identify individual components among simple protein mixtures [60, 61]. Improved sample preparation methods also provided an enhanced sensitivity to enable analysis of femtomolar levels of protein [5, 19], Today, the automation of MALDI-MS data acquisition enables the analysis of hundreds of samples each day in large-scale protein identification experiments [62]. 

Large-scale protein identification by 2D-LC-MS/MS is also referred to as multidimensional protein identification technology (MudPIT) [73,74,220]. With this approach, protein identification relies solely on MS/MS data. It has been shown, however, that including the LC retention time as peptide-specific information can improve the quality of protein identification significantly. 

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