Large high-quality crystals

Cocrystallization and the Formation of Large High Quality Crystals... 

For application of X-ray diffraction to a macromolecule, the protein or nucleic acid must first be crystallized. Not only must crystals be grown, but they must be reasonably large, high-quality crystals that are suitable for a high-resolution X-ray diffraction study. The crystallization step has emerged as the primary obstacle in macromolecular crystallography. This is principally due to the empirical nature of the methods employed to overcome it, and the complexity of the molecules involved (McPherson, 1976, 1982, 1999). 

Beyond the abundant natural sources of quartz and other silicas, techniques for synthetic production of these materials have provided a significantly wider range of applications [27,34], Large, high-quality crystals of quartz can be grown by the well-established technique of hydrothermal growth in an autoclave filled with a solution of... 

Study of CDW instabilities in blue bronze is facilitated by the ease preparation of large, high-quality crystals by electrolysis of K2M0O4/M0O3... 

The ADtcp complexes are the most extensively studied series of Krogmann salts. In particular, the solid state properties of K2[Pt(CN)4]Br0.30 3.2H2O, KCP(Br), have been studied in great detail because of the availability of large high quality single crystals.30-32... 

In the course of preparing cocrystals of nitroaniline dimers and of related compounds from solution we observed that large high-quality cocrystals often form even when one or both of the component molecules give poor crystals by themselves. This observation... 

X-ray diffraction (crystallography) has been around for much longer than NMR and has been used to determine the precise three-dimensional stmcture of biomolecules as large as viruses (molecular weight in the tens of millions ). This technique requires a high-quality crystal and calculates a three-dimensional map of electron density for the molecule. The... 

For a satisfactory crystallographic study, it is vital to start with a high quality crystal. This is not easy for proteins. Crystallisation of such large molecular complexes frequently leads to trapped solvent, and a reasonable protein crystal may still contain between 30-80% solvent. Because the... 

Structure of the myosin heads. Myosin and myosin fragments can be isolated in large quantities, but they have been difficult to crystallize. However, Payment and coworkers purified SI heads cleaved from chicken myosin by papain and subjected them to reductive methylation (using a dimethylamine-borane complex see also Eq. 3-34). With most of the lysine side chain amino groups converted to dimethylamine groups, high-quality crystals were obtained, and a structure was determined by X-ray diffraction. ... 

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