Lanthanide-binding tags

G. Site-specific labeling of proteins with a rigid lanthanide-binding tag. ChemEioChem 2006, 7,1599-1504. 

Franz, K.J., Nitz, M., and Imperiali, B. (2003) Lanthanide-binding tags as versatile protein coexpression probes. Chem. Bio. Chem. 4, 265-271. 

Martin, L.J., Sculimbrene, B.R., Nitz, M., and Imperial/ B. (2005) Rapid combinatorial screening of peptide libraries for the selection of lanthanide-binding tags (LBTs). QSAR Combin. Sci. 24(10), 1149-1157. 

Sculimbrene, B.R., and Imperiali, B. (2006) Lanthanide-binding tags as luminescent probes for studying protein interactions./. Am. Chem. Soc. 128(22), 7346-7352. 

Martin LJ, et al. Double-lanthanide-binding tags design, photophysical properties, and NMR applications. J. Am. Chem. Soc. 41. 

An introduction of a coexpressed lanthanide-binding tag (LBT) as a method for protein alignment facilitating the measurement of RDCs has been proposed by Wohnert et The LBT chosen by them had the sequence YID-TNNDGWYEGDELLA, and was appended to the terminus of human ubiquitin by standard cloning techniques. 

Barthelmes et al have inserted different length lanthanide-binding tags (used for RDCs measurements) into three loop regions of interleukin-l S and shown that the insertion does not alter the X-ray structure of the protein. 

LBT peptide equence, the aminoacid bearing the sensitiza side-chain is denoted X Fig. 4 Lanthanide-binding tags (LBTs)... 

Fig. 6 Structure of human galectin-3 CRD in complex with a lactose moiety bearing a lanthanide binding tag. Significant chemical shift perturbations (PCS) in the protein (residues represented as sticks) can be detected upon ligand binding (modelled from galectin structure PDB 2NN8).

Barthelmes K., A. M. Re3molds, E. Peisach, H. R. A. Jonker, N. J. DeNunzio, K. N. Allen, B. Imperiali, H. Schwalbe. Engineering Encodable Lanthanide-Binding Tags into Loop Regions of Proteins, J. Am. Chem. Soc., 133, 808-819 (2011). 

---