Laminar airflow workstations

After the cycle, aseptically transfer the spore strip to vessels of culture media. If spore suspensions were used, aseptically transfer the inoculated bottles to a laminar airflow workstation and add culture media to the bottles. Use appropriate positive and negative controls. 

Terminally sterilized products Solutions should generally be prepared in a grade C environment in order to give low microbial and particulate counts, suitable for immediate filtration and sterilization. Solution preparation could be allowed in a grade D environment if additional measures were taken to minimize contamination, such as the use of closed vessels. For parenterais, Ailing should be done in a laminar-airflow workstation (grade A) in a grade C environment The preparation of other sterile products,... 

The terminal HEPA filtration module (TFM) is a self-contained HEPA filter and plenum unit (Fig. 11), which may be used to provide laminar or conventional airflow to a clean space, or may be dedicated as a LAF workstation.f The TFM is available with a 10 in. (optional 12 inch) collar for connection by a circular supply duct to a central air handling system (Fig. 5A) or as a free-standing, fully powered unit containing a motor and blower. It is normally installed in a... 

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