Laccase activity assay

Enzyme Assays. Laccase activity was determined by oxidation of 2,2-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS). The reaction of suitably diluted enzyme was determined at 420 nm in the presence of 0.03% ABTS and 100 mM sodium acetate buffer, pH 5.0. The extinction coefficient of ABTS is 420 = 3.6 x 104 M-1 cm-1 (26). 

Alternate Protocol 2 Spectrophotometric Assay of Laccase Activity C4.1.8... 

Laccase activity, diphenyl oxidase assays, 394, 396, 398 (table)... 

The titre of antibodies in laccase antisera was 1 16 as measured by the immunodiffusion technique of Ouchterlony (21). These antibodies were effective inhibitors of catechol oxidase activity, with 100/il of antisera reducing activity by 95%, in an assay mixture of 3ml of 0.1M catechol in... 

Similar to laccase, a number of methods have been developed to engineer GOx for increased activity and specificity. An enzymatic assay coupled with site-directed mutagenesis was used to alter the substrate specificity of GOx to increase the dynamic range of glucose biosensors [34], and a directed evolution screen was developed to improve GOx activity in BFCs [35]. 

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