Label-Free Electrochemical Genosensing

Label-free genosensing techniques have a great importance for sequence specific detections in pharmaceutical and environmental 

A strong DNA immobilization method was developed by using chitosan which is a cationic polymer that forms polyelectrol3d e 

Sequence-specific bioelectronic detection of PCR amplicons were performed with unpurified PCR samples by Lai et al [86]. GyrB genes of Salmonella typhimurium were produced in PCR reaction and detection was performed by applying AC voltammetry. Manalis group investigated a label-free microelectronic PCR quantification [87], A field-effect microelectronic sensor was developed which was capable of quanification of DNA during PCR reaction at polylysine covered surfaces. 

Direct bioelectronic detection of multiple point mutations in Mycobacterium tuberculosis amplicons related to rifampin drug resistance was perfomed [98]. In recent studies, it was found that 95% of RIF-resistant bacteria strains possess mutations within 

Throughout this chapter, we demonstrated label-based and label-free electrochemical genosensing techniques for the detection of microbiological and inherited diseases devoted to clinical analysis. The sensor technology is relatively cheap to produce, easily 

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A. Erdem, M.I. Pividori, M. Del Valle and S. Alegret, Rigid carbon composites a new transducing material for label-free electrochemical genosensing, J. Electroanal. Chem., 567 (2004) 29-37. 

Figure 13.5. Schematic Illustration of electrochemical label-free allele-specific genosensing method.

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