Label-free detection methods surface plasmon resonance

The surface plasmon resonance (SPR) is the analytical technique widely applied in the field of characterizing adsorption to surfaces. The big advantage of this method is the high sensitivity in detecting substances adsorbed on a surface (Ipg/pm of protein molecules) and the possibility of label-free measurements also in the in situ mode. The resulting graph is presented in the arbitrary units and called sensogram. 

Label-fi ee strategies provide an alternative route for circumventing the above-mentioned difficulties with respect to protein microarray detection. Currently, relatively few label-fi-ee detection methods such as mass spectrometry (MS) (2,3), surface plasmon resonance (SPR) (4) and atomic force microscopy (AFM) (5,6) are applicable to the microarray format. These label-free technologies require the use of sophisticated tools that are not available in all bioanalytical laboratories. 

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