L-Iditol dehydrogenase

Enzymic syntheses are considered next. Xylitol is a substrate for sheep-liver L-iditol dehydrogenase, a NAD-linked enzyme. 1-Deoxy-D-xylitol, prepared by Raney nickel reduction of D-xylose diethyl dithioacetal in a 27% overall yield from D-xylose, was also reported31 to be a substrate, although with a higher Km and lower Vmax. The product was assumed to be l-deoxy-D-f/ireo-pentulose because of the appearance of a yellowish fluorescent spot when a chromatogram was sprayed with acidic 3,5-aminobenzoic acid, resembling that formed from 1 -deoxyfructose. There was no more-rigorous characterization. 

EC 1.1.1.14 L-Iditol dehydrogenase EC 1.1.1.27 Lactate dehydrogenase L-Iditol + NAD + t L-sorbose + NADH Lactic acid + NAD+ pyruvic acid + NADH + H + — SM... 

D-Xylose Isomerases.—An enzymic method that employs a commercial preparation of L-iditol dehydrogenase containing D-xylulose reductase activity has been reported for the assay of o-xylose isomerase. ... 

A review on the preparation and separation of inositol tetrakisphosphates and the establishment of enantiomeric configuration by use of L-iditol dehydrogenase has appeared. The synthesis and biological evaluation of inositol derivatives as inhibitors of phospholipase C (PLC) has been reviewed. A review on the chemistry, preparation and enzymatic reactions of inositol phosphates and a review on the biochemistry, stereochemistry and nomenclature of inositol phosphates has also been reported. A part review on phosphonate analogues of myo-inositol triphosphate has appeared. ... 

Reagents i, DHAP, fructose 1,6-diphosphate aldolase ii, phosphatase iii, L-iditol dehydrogenase iv, H3O ... 

This enzyme [EC 1.1.1.14], also known as L-iditol 2-dehydrogenase and polyol dehydrogenase, catalyzes the reaction of L-iditol with NAD+ to produce L-sorbose and NADH. The enzyme will also convert D-glucitol to D-fructose. 

D-Glucitol dehydrogenase has broad substrate-specificity. The enzyme oxidizes D-glucitol, L-iditol, ribitol, and xylitol in the presence of NAD+ cofactor.414 424 NAD+ can be replaced by 3-acetylpyridine adenine dinucleotide (AcPyAD), 3-thionicotinamide adenine dinucleotide (TNAD), or nicotinamide hypoxanthine dinucleotide (NHD).414... 

Polyol dehydrogenase. Sorbitol dehydrogenase. L-iditol + NAD(+) = L-sorbose + NADH. 

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