L-Glutamate decarboxylase

D-Glutamic acid has been detected in samples of poly-a,L-glutamic acid (PGA) irradiated in 1% solution at pH 6.8 (Na salt). The D-glutamic acid was measured in hydrolyzates of the polymer by an enzymic method 36). A specific enzyme, L-glutamate decarboxylase E. coli), converts L-glutamic... 

L-Glutamate Glutamate dehydrogenase, l-glutamate decarboxylase, l-glutamate oxidase Fruits, juices, wines, ciders Flavour enhancer... 

Oliveira et al. [32] L-Glutamate Commercial soups L-Glutamate decarboxylase/ in glass beads Potentiometric ionic sensor ... 

Lin C-T, Li J-Z, Wu J-Y (1983) Immunocytochemical localization of L-glutamate decarboxylase, gamma-aminobutyric acid transaminase, cysteine sulfinic acid decarboxylase, aspartate aminotransferase and somatostatin in rat retina. Brain Res 270 273-283. 

As early as 1975, Ahn et al. developed an enzyme electrode for glutamate composed of L-glutamate decarboxylase (EC 4.1.1.15) and a carbon dioxide sensor. 

Subsequent to this work, apoaspartate transaminase was used to assay the stereospecificity of a variety of other transaminases, all of which were shown to involve protonation/deprotonation at the C-4 Si face of the cofactor. These enzymes included pyridoxamine-pyruvate transaminase (EC 2.6.1.30) (26) and a-dialkylamino acid transaminase (27). L-Glutamate decarboxylase (EC 4.1.1.15) catalyzes an abortive transamination reaction when oc-methylglutamate is used as substrate, and this too was shown to occur with protonation at the Si face of C-4 in the intermediate 4d (28) as was the abnormal transamination of D-alanine by serine hydroxymethyltransferase (29). 

Denner L. A and Wu J -Y (1985) Purification and characterization of L-glutamate decarboxylase from whole rat brain J Neurochem, (In Press). 

Wu J -Y (1976) Purification and properties of L-glutamate decarboxylase (GAD) and GABA-aminotransferase (GABA-T), in GABA m Nervous System Function (Roberts E, Chase T. and Tower D., eds.), pp 7-55. Raven Press, New York, N Y. 

Wu J.-Y. (1982) Purification and characterization of cysteic/cysteine sulfinic acids decarboxylase and L-glutamate decarboxylase in bovine brain Proc Natl Acad. Sci. USA 79, 4270-4274... 

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