L-a-glycerophosphorylcholine

L-a Glycerophosphorylcholine cadmium chloride complex was prepared by hydrolyzing egg lecithin which was isolated from egg yolk. 1-Dodecyne, undecylenic acid, 11-bromo undecanoic acid, nonanoic acid, lithium acety-lide ethylenediamine complex, dimethylaminopyridine (DMAP), and dicyclo-hexylcarbodiimide (DCC) were purchased from Aldrich Chemical Co. Milwaukee, WI. Tridecyne was obtained from Farchan Laboratories, Gainesville, FL, and 1,2-dihexanoyl and 1,2-dipalmitoyl PC were purchased from Avanti Polar Lipids, Birmingham, AL. Mixed bed resin AG 501-X8(D) was procured from Bio-Rad Laboratories, Richmond, CA. Chloroform was distilled freshly over phosphorus pentoxide just before the use. Other solvents were used as received. The water was triply distilled and deionized. 

HeLa cells are derived from a human cancer and maintained in tissue culture. Evans and Kaplan (1977) have carried out a survey of the phosphorus compounds present in HeLa cells and their extracts using P NMR spectrometry at 40 MHz. The proton-decoupled P spectrum of neutralized perchloric acid extracts has sufficient resolution to allow the main phosphate compounds to be identified The observed composition, in order of decreasing concentration, was inorganic phosphate, ATP, phosphoryl-choline, creatine phosphate, UTP, NAD, glucose-6-phosphate, J -D-fruc-tose-l,6-bisphosphate, ADP, a-glycerophosphorylcholine, and a-glycero-... 

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