Kyowa Hakko Kogyo Co. Ltd

H. Tanaka and K. Taniguchi (Kyowa Hakko Kogyo Co., Ltd., Japan), PCTInt. Appl. WO 2006009093 (2006) (Chem. Abstr.,... 

Professor Hiroshi Okximoto of Kyoto University for supplying rice seeds. Professor Toshiaki Mitsui of Niigata University for supplying rice cells, and Kyowa Hakko Kogyo Co., Ltd., Tokyo, Japan for the gift of ABA. 

Figure 5 The production systems for globotriose (Galal,4Lac) [69] and A -acetyllactosamine [70] as designed by researchers at the Tokyo Research Laboratories, Kyowa Hakko Kogyo Co. Ltd. Both IgtB and IgtC were from Neisseria gonorrhoeae.

Figure 1 Elution profile of 2.5 mg l-ASP preparations on a TSK SW 4000 blue column (Pharmacia-LKB, 7.5 x 60 mm). The flow rate was 60 mL/h each fraction contained 0.5 mL. Protein concentration is recorded automatically. The enzyme activity (U/mL) was determined in each individual fraction. Molecular weight markers are indicated by arrows, (a) Crasnitin from Bayer, Leverkusen, FRG (b) Leunase from Kyowa, Hakko, Kogyo Co. Ltd., Tokyo, Japan (c) Erwinia-Asp from The Centre for Applied Microbiology, Salisbury, U.K. (d) PEG-modified E. coli 1-ASP from ENZON, South Plainfield, NJ.

New glycols were developed, which had hydrophobic groups and relatively high steric hindrances, which were used to improve the hydrolysis resistance of polyester-based PU - 2-butyl-2-ethyl 1,3 propanediol (BEPG Kyowa Hakko Kogyo Co. Ltd.), (see Figure 8.2). 

Ikeda S et al (Kyowa Hakko Kogyo Co. Ltd.) Propenone derives. JP 96277242. 

Production of L-aspartic acid from fumaric acid by stereoselective addition of ammonia under the action of the intracelluar aspartase in E. coli (Tanabe Seiyaku Co., Ltd.). When a 1000-liter column is used, theoretical yield of L-aspartic acid is 3.4 tons per day (and even considerably higher for mutant strains and plasmid pNKl01-harboring strains). A similar industrial process using the immobilized E. coli aspartase (instead of the whole cells) was established earlier by Kyowa Hakko Kogyo, Co., Ltd.. L-Aspartate is mainly used as a building block for the manufacture of the sweetener aspartame [170]. 

Y. Soma, N. Yamamoto, H. Sano, K. Yamauchi, K. Tamaoki, K. Tanaka and T. Yabushita (Agency of Industrial Sciences and Technology, Japan, Kyowa Hakko Kogyo Co. Ltd.) ... 

Research in the authors laboratory is suf rted by grants BES-9816490 and BES-9604561 from the National Science Foundation, the Schlumbeiger Fellowship of DSC, and by Kyowa Hakko Kogyo Co. Ltd. We thank Peter C. Michels for assistance in preparing the manuscript. 

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