Kinetics of Enzyme-Catalysed Biochemical Reactions

Complex ES is assumed to be in a stationary state as the rate of formation of ES is equal to the rate of dissociation of ES. Thus 

The total amount of enzyme Eg is fixed as there is no net consumption of enzyme. Thus, total concentration of enzyme. Eg, is the sum of concentrations of free enzyme [E] and bound enzyme [ES]. 

On insertion of Equation 2.168 in Equation 2.164, we get the rate equation for the enzyme-catalysed reaction as 

Rate rp versus substrate concentration [S] for enzyme-catalysed reaction. 

There are some enzyme-catalysed reactions in which the substrate [S] inhibits the formation of product P by binding to the energised complex ES to form a complex ES2. The mechanism of this reaction is represented as 

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