Kinetics bacterial luciferase

Abu-Soud, H., Mullins, L. S., Baldwin, T. O., and Raushel, F. M. (1992). Stopped-flow kinetic analysis of the bacterial luciferase reaction. Biochemistry 31 3807-3813. 

Bioluminescence-based analytical assays were used to measure various analytes in nanoliter sample volumes. Nanoliter volumes of multiple bioluminescent analytical assays were deposited in an array format and lyophilized. ATP-firefly luciferase (FFL) and NADH-bacterial luciferase (BL) platform reactions were compared. We achieved parallel sample delivery via sample-hydrated membranes. A CCD camera measured the luminescent kinetics for each assay. These miniaturized assays and instruments can he prepared as micro-analytical systems to operate in point-of-care (POC) diagnostic devices. 

The kinetic mechanism will be confined to V. harveyi luciferase, the most extensively studied bacterial luciferase to date. Our version of the kinetic scheme is shown in Scheme 136.6. Aside fi-om the aldehyde... 

Francisco, W.A., Abu-Soud, H.M., DelMonte, A.J., Singleton, D.A., Baldwin, TO., and Raushel, F.M., Deuterium kinetic isotope effects and the mechanism of the bacterial luciferase reaction. 

Holzman, T.F. and Baldwin, T.O., Reversible inhibition of the bacterial luciferase catalyzed bioluminescence reaction by aldehyde substrate kinetic mechanism and Ugand effects. Biochemistry, 22, 2838, 1983. 

Thoden, J.B., Holden, H.M., Fisher, A.J., Sinclair, J.F., Wesenberg, G., Baldwin, TO., and Rayment, 1., Structure of the Pj homodimer of bacterial luciferase from Vibrio harveyi x-ray anal) is of a kinetic protein folding trap. Protein Sci., 6, 13,1997. 

Eckstein, J. W., Hastings, J. W., and Ghisla, S. (1993). Mechanism of bacterial bioluminescence. 4a,5-Dihydroflavin analogs as models for luciferase hydroperoxide intermediates and the effect of substituents at the 8-position of flavin on luciferase kinetics. Biochemistry 32 404 111. 

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