Kinetic parameter estimation light-scattering measurements

Witkowski, W.R., Miller, S.M. and Rawlings, J.B., 1990. Light scattering measurements to estimate kinetic parameters of crystallization. In Crystallization as a separation process, ACS Symposium Series, 438, 102. 

Light-Scattering Measurements To Estimate Kinetic Parameters of Crystallization... 

It is shown that while solute concentration data can be used to estimate the kinetic growth parameters, information about the CSD is necessary to evaluate the nucleation parameters. The fraction of light obscured by an illuminated sample of crystals provides a measure of the second moment of the CSD. Numerical and experimental studies demonstrate that all of the kinetic parameters can be identified by using the obscuration measurement along with the concentration measurement. It is also shown that characterization of the crystal shape is very important when evaluating CSD information from light scattering instruments. 

The bio-activity of synthesized samples was estimated from mobility parameters of unicellular microorganisms measured by Dynamic Light Scattering (DLS). The following characteristics of cell population were measured cell concentration (units/ml) % of mobile cells mean translation velocity (pm/s), cell rotational frequency of (Hz) flagella beat frequency (Hz), distribution of beat frequencies, and kinetic energy of cell motion in a viscous environment (arbitrary units). Details of experimental equipment and measurement procedures may be found elsewhere.7,8 All parameters were measured with an accuracy of about 2%.9... 

---