Kinetic models for the transport process

These relationships are identical to Haldane relationships, but unlike the latter, their validity does not derive from a proposed reaction scheme, but merely from the observed hyperbolic dependence of transport rates upon substrate concentration. Krupka showed that these relationships were not obeyed by the set of data previously used by Lieb [64] to reject the simple asymmetric carrier model for glucose transport. Such data therefore cannot be used either to confirm or refute the model. 

Measurements of individual rate constants for steps in the transport cycle 

Assuming that the simple, four-state asymmetric carrier model does accurately describe the transport process, Lowe and Walmsley [48] have exploited the tempera- 

The Kmax (and K, see below) constants determined from steady-state kinetic measurements are thus seen to be complex constants containing two or more of the individual rate constants illustrated in Fig. 2. 

Rate constants governing re-orientation of the glucose transporter, and their activation energies, determined from steady-state and pre-steady-state measurements 

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