Kinase ligand immobilization

Where a preferential enzyme substrate, e.g. for a protein kinase, is to be enriched, it is only the modified (e.g. phosphorylated phage, Ref. 42) phage which is selected. This modified ligand phage can, for instance, be enriched on an antibody, which can be immobilized in vast excess. In this case the enzyme need only be present in trace catalytic amounts, and indeed a limiting concentration of the enzyme is necessary to distinguish the preferred substrate for promiscuous kinases [43],... 

Shinohara, H. Matsubayashi, Y Functional immobilization of plant receptor-like kinase onto miCTobeads towards receptor array construction and receptor-based ligand fishing. Plant J. 2007, 52, 175-184. 

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