Kieselguhr G, Impregnated with pH 5 Phosphate Buffer

Kieselguhr G (20 g) is mixed with 40 ml of pH 5 phosphate buffer (prepared by mixing equal volumes of 0.1 M phosphoric acid and 0.1 M disodium hydrogen phosphate). The plates are spread to a thickness of 250 fx and dried overnight in the air. From 5 to 25 [xg of carbohydrate is applied to the starting point. 

The plates are prepared as described in [1] using 60 ml of the following solutions instead of water  

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