2-Ketoglutarate 2,4-dichlorophenoxyacetate

Matheson VG, LJ, Forney, Y Suwa, CH Nakatsu, AJ Sextone, AJ, WE Holben (1996) Evidence for acquisition in nature of a chromosomal 2,4-dichlorophenoxyacetic acid/a-ketoglutarate dioxygenase gene by different Burckholderia spp. Appl Environ Microbiol 62 2457-2463. 

Fukumori F, RP Hausinger (1993) Alcaligenes eutrophus IMP 134 2,4-dichlorophenoxyacetate monooxygenase is an a-ketoglutarate-dependent dioxygenase. J Bacterial 175 2083-2086. 

Sassanella TM, F Fukumori, M Bagdasarian, RP Hausinger (1997) Use of 4-nitrophenoxyacetic acid for detection and quantification of 2,4-dichlorophenoxyacetic acid 2,4-D/a-ketoglutarate dioxygenase activity in 2,4- D-degrading microorganisms. Appl Environ Microbiol 63 1189-1191. 

Suwa, Y., W.E. Olben, and L.J. Forney. 1996. Characterization of chromosomally encoded 2,4-dichlorophenoxyacetic acid-a-ketoglutarate dioxygenase from Burkholderia sp. strain RASC. Appl. Environ. Microbiol. 62 2464-2469. 

The 2,4-dichlorophenoxyacetic acid/a-ketoglutarate dioxygenase could accept 4-nitrophenoxyacetic acid to produce colored 4-nitro-phenol (Sassanella et al. 1997). It was pointed out, however, that a number of strains that were able to degrade 2,4-dichlorophenoxy-acetic acid were unable to accept the surrogate substrate. 

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